Antifungal susceptibility of clinically significant candida species by disk diffusion method

Original Article

Author Details : Ajitha Reddy Edula*

Volume : 7, Issue : 2, Year : 2021

Article Page : 77-80

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Context: Antifungal susceptibility of candida.
Aims: To perform antifungal susceptibility testing on candida isolates by disk diffusion method & study its susceptibility pattern.
Settings and Design: The present study was conducted in the department of Microbiology in a tertiary care hospital in Hyderabad from January 2013 to June 2014, with prior approval of the Institutional Ethics Committee. The present study was designed to perform antifungal susceptibility test on Candida isolates by Disk Diffusion Method and study its susceptibility pattern.
Materials and Methods: 102 Candida isolates were subjected to Antifungal susceptibility testing by Disk diffusion method using Mueller-Hinton Agar + 2% Glucose and 0.5 g/mL Methylene Blue Dye (GMB) Mediumas per CLSI guidelines.
Statistical analysis used:
Results: Antifungal susceptibility test shows that C. albicans is more susceptible to all the antifungal agents tested. Resistance to azole group of drugs was more pronounced in non-albicans candida spp. Voriconazole seemed to be superior to Fluconazole with a better susceptibility in the Fluconazole resistant strains also.
Conclusions: Findings of the antifungal susceptibility test suggest that Candida spp., differ in their susceptibility to antifungal agents. Antifungal susceptibility testing of Candida isolates will be helpful in guiding physicians to select the appropriate antifungal drug so that therapeutic failures can be avoided thus decreasing patient morbidity and mortality.

Keywords: Candida, Antifungal susceptibility, Disk diffusion method.

How to cite : Edula A R, Antifungal susceptibility of clinically significant candida species by disk diffusion method. IP Int J Med Microbiol Trop Dis 2021;7(2):77-80

Copyright © 2021 by author(s) and IP Int J Med Microbiol Trop Dis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (

Article History

Received : 28-04-2021

Accepted : 21-05-2021

Available online : 25-06-2021

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