Biofilm production and antibiotic resistance pattern among bacterial isolates from wound samples in a rural tertiary care teaching hospital

Original Article

Author Details : Venkatesha D, Dhanalakshmi T. A, Shakthi R*

Volume : 7, Issue : 3, Year : 2021

Article Page : 160-164

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Introduction: Pyogenic wound infections are the one of the leading cause of morbidity and mortality worldwide. Some of the common etiological agents responsible are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Klebsiella app., Proteus spp., Pseudomonas spp., and Acinetobacter spp. The antimicrobial resistance has become a global challenge and the resistant pathogen poses a grave threat to the public health worldwide. Pyogenic bacteria producing biofilm has a potential to cause significant mortality and morbidity in human.
Aim: The present study was carried out to determine the bacteriological spectrum of wound infections and their antibiogram to commonly used antibiotics and to detect the biofilm production by the isolates. 
Materials and Methods: This cross sectional study was carried out in the department of Microbiology, Adichunchanagiri institute of Medical sciences from September 2016 to August 2017. Two hundred and forty samples from various wounds were collected and processed as per standard procedures and biofilm production was detected by Congo red agar method.
Results: Out of 240 pus isolates, Staphylococcus species were the most commonly isolated (48.85%) followed by Pseudomonas species (11.7%). Biofilm was produced by 49.2% isolates .Majority of Gram negative bacilli were susceptible to Colistin (100%) followed by Tigecycline (Biofilm producers 75%, biofilm non producers 66.7%).All Gram positive isolates were susceptible to Vancomycin and Teicoplanin (100%) followed by Linezolid (biofilm producer 98.8%, biofilm non producer 97.8%) .
Conclusion: Routine surveillance for wound infections along with early identification and adopting efficient control protocol against biofilm forming organism plays an important role in the prevention of the most serious infections.

Keywords: Antibiotic resistance, Biofilm, Congo red agar, Wound infection

How to cite : Venkatesha D, Dhanalakshmi T. A, Shakthi R, Biofilm production and antibiotic resistance pattern among bacterial isolates from wound samples in a rural tertiary care teaching hospital. IP Int J Med Microbiol Trop Dis 2021;7(3):160-164

Copyright © 2021 by author(s) and IP Int J Med Microbiol Trop Dis. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (

Article History

Received : 29-06-2021

Accepted : 02-08-2021

Available online : 01-09-2021

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