When microorganisms are tested, the laboratories provide good conditions for the growth of microbes. Therefore, there is a need to develop techniques to manage areas of high microbial populations and mechanisms to prevent those entering aseptically protected areas like media preparation room. So the basic design of a microbiology laboratory needs to include the physical separation of spaces to carry out different functions and requirements. Media room is the basis of a microbiology laboratory, so it needs special attention. The present paper reviews the design and proper functioning of an ideal media preparation room.