Study on occurrence of Hepatitis B virus Genotypes at a tertiary care hospital in Jaipur

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Original Article

Author Details : Ruby Naz*, Mohammad Khalid Farooqui, Aditi Gaur, Bharti Malhotra

Volume : 10, Issue : 3, Year : 2024

Article Page : 276-283

https://doi.org/10.18231/j.ijmmtd.2024.047

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Abstract

Introduction: The hepatitis B virus’s (HBV) capability to mutate genetically poses a threat to the sensitivity of serological and genomic diagnostics. As a result the knowledge about the distribution of HBV genotypes (GENs) and mutation has a significant impact on treatment plans, immunisation campaigns, treatment, and prophylaxis of HBV.
Aim and Objectives: The objective of our study was to identify HBV Genotypes in HBsAg-positive individuals.
Materials and Methods: The study was conducted on 117 HBsAg-positive patients who showed the presence of the S gene were subjected to DNA extraction. The extracted DNA was amplified by Nested PCR (Polymerase Chain Reaction) followed by RFLP (Restriction fragment length polymorphism).
Results: Out of 117 patients, 84% were genotype D and 16 % were genotype A. More patients of Genotype A had a fever and Jaundice (15.7%; 21 vs 5%; 13.2%) as compared to Genotype D. In this study 19.6% of patients were in the immune clearance phase, 18% in the inactive carrier, 6% in immune tolerance phase.
Conclusion: A better understanding of the prevalence, diagnostic, and serologic patterns of hepatitis B in this region can aid practitioners in recommending appropriate antivirals by genetic data.

Keywords: HBV, Nested PCR, gene, DNA, Extraction HBV Genotype, immunopathogenesis of HBV, RFLP

How to cite : Naz R, Farooqui M K, Gaur A, Malhotra B, Study on occurrence of Hepatitis B virus Genotypes at a tertiary care hospital in Jaipur. IP Int J Med Microbiol Trop Dis 2024;10(3):276-283

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